Assessment of potential toxicity of a smokeless tobacco product (naswar) available on the Pakistani market
- Muhammad Saeed1,
- Naveed Muhammad1,
- Saeed Ahmad Khan1,
- Farah Gul1,
- Fazli Khuda1,
- Muhammad Hamuyun2,
- Hamayun Khan1
- 1Department of Pharmacy, University of Peshawar, Peshawar, Pakistan
- 2National Centre of Excellence in Geology, University of Peshawar, Peshawar, Pakistan
- Correspondence to Dr Zakiullah, Department of Pharmacy, University of Peshawar, Peshawar, Pakistan;
Contributors Zakiullah was project manager, performed pH analysis, 100% nicotine analyses and was author of the first draft. Muhammad Saeed was study originator, and was involved in writing/editing of the. Naveed Muhammad performed heavy metals analyses. Saeed Ahmad Khan coordinated sample acquisition. Fazli Khuda was involved with extensive writing/editing of the paper. Farah Gul performed sample preparation for nicotine and pH analysis. Muhammad Humayun performed nitrate and nitrite analysis. Hamayun Khan performed extensive writing/editing of the paper.
- Received 26 December 2010
- Accepted 20 April 2011
- Published Online First 3 June 2011
Background ‘Naswar’ is a smokeless tobacco product (STP) widely used in Pakistan. It has been correlated with oral and oesophageal cancer in recent clinical studies. The toxic effects associated with STPs have been associated with trace level contaminants present in these products. The toxin levels of Pakistani naswar are reported for the first time in this study.
Methods A total of 30 Pakistani brands of naswar were tested for a variety of toxic constituents and carcinogens such as cadmium, arsenic, lead and other carcinogenic metals, nitrite and nitrate, and nicotine and pH.
Results The average values of all the toxins studied were well above their allowable limits, making the product a health risk for consumers. Calculated lifetime cancer risk from cadmium and lead was 1 lac (100000) to 10 lac (1000000) times higher than the minimum 10E-4 (0.00001) to 10E-6 (0.000001), which is the ‘target range’ for potentially hazardous substances, according to the US Environmental Protection Agency. Similarly, the level of arsenic was in the range of 0.15 to 14.04 μg/g, the average being 1.25 μg/g. The estimated average bioavailable concentration of arsenic is 0.125–0.25 μg/g, which is higher than the allowable standard of 0.01 μg/g. Similarly, the average minimum daily intake of chromium and nickel was 126.97 μg and 122.01 μg, as compared to allowable 30–35 μg and 35 μg, respectively; a 4–5 times higher exposure. However, beryllium was not detected in any of the brands studied. The pH was highly basic, averaging 8.56, which favours the formation of tobacco specific amines thus making the product potentially toxic. This study validates clinical studies correlating incidence of cancer with naswar use in Pakistan.
Conclusions This study shows that the production, packaging, sale and consumption of naswar should be regulated so as to protect the public from the health hazards associated with its consumption.
Funding Funding was received from the University of Peshawar, Peshawar, Pakistan.
Competing interests None.
Provenance and peer review Not commissioned; externally peer reviewed.