Little information exists on the various measures used to assess tobacco withdrawal. This article reviews the literature on the assessment of tobacco withdrawal, specifically comparing three commonly used self-report questionnaires of tobacco withdrawal. Several topics are discussed including (a) the frequency and duration of withdrawal assessment, (b) the determination of reliability and validity of self-report measures, (c) a comparison of the existing self-report instruments in terms of psychometric properties and other criteria, and (d) the physiological and behavioral assessment of tobacco withdrawal. Six recommendations for further research are offered.