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Waterpipe tobacco smoke toxicity: the impact of waterpipe size
  1. Cindy DeForest Hauser1,
  2. Ronnae Mailig1,
  3. Hannah Stadtler2,
  4. Jenna Reed2,
  5. Shi Chen2,
  6. Emilie Uffman2,
  7. Karen Bernd2
  1. 1Department of Chemistry, Davidson College, Davidson, North Carolina, USA
  2. 2Department of Biology, Davidson College, Davidson, North Carolina, USA
  1. Correspondence to Dr Karen Bernd, Department of Biology, Davidson College, Davidson, NC 28035, USA; kabernd{at}davidson.edu

Abstract

Introduction Waterpipe tobacco smoking continues to show increasing popularity, especially among individuals between 18 and 22 years old. Waterpipe tobacco smoke (WTS) is a mixture of particulates and gases formed from the combustion of the charcoal and volatilisation and humidification of the tobacco+humectant+flavouring substrate known as shisha or mu’assel. As such, variation in the configuration of the waterpipe may affect the particles produced. Our study focuses on the effects of waterpipe size on the physical properties and cytotoxicity of the smoke produced.

Methods Shisha type and headspace volume were held constant and a modified Beirut puff protocol was followed while the size of the waterpipe was varied. Particle concentrations and size distributions were measured using a TSI Engine Exhaust Particle Sizer. Type II alveolar cells were exposed to smoke at the air-liquid interface and two metrics of cell health analysed.

Results In a 30 min session, we observed a decrease in total particle concentration (1014–1013) and mass (10 000–2800 mg/m3) and an increase in particle size (125–170 nm) as pipe height increases from 22 to 55 cm and bowl size from 300 to 1250 mL. Smoke from all pipe sizes caused decreases in lysosomal function (>40%) and membrane integrity (>60%) 24 hours post 57 min exposure, and meet the National Institutes of Health definition of a cytotoxic agent (≥30% decrease in cell viability).

Conclusion Smoke from waterpipes of all sizes causes significant alveolar cellular harm, indicating that this device needs regulation as a hazard to human health.

  • waterpipe
  • shisha
  • particulate matter
  • air-liquid interface
  • waterpipe tobacco smoke
  • toxicity
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Footnotes

  • Contributors CDFH and KB are the guarantors. CDFH and KB conceived the experimental design and drafted the manuscript. RM and CDFH performed the physical characteristic studies, data analysis and figure preparation. HS, JR, SC, EU and KB performed the cell exposure studies, data analysis and figure preparation.

  • Funding Research reported in this publication was supported by grant number R01HL134169 from the NIH National Heart, Lung, and Blood Institute and FDA Center for Tobacco Products.

  • Disclaimer The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH or the Food and Drug Administration.

  • Competing interests None.

  • Patient consent for publication Not required.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement Data are available upon reasonable request.

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