%0 Journal Article %A Amira Yassine %A Rachel El Hage %A Ahmad El-Hellani %A Rola Salman %A Soha Talih %A Thomas Eissenberg %A Alan Shihadeh %A Najat Saliba %T Levels of menthol, nicotine and cooling agents measured in JUUL products purchased across a three-year period %D 2022 %R 10.1136/tc-2022-057506 %J Tobacco Control %P s234-s237 %V 31 %N Suppl 3 %X Background In response to the popularity of JUUL and other flavored ENDS products among youth, the US Food and Drug Administration issued in February 2020 an enforcement policy to remove all flavoured cartridge/pod-based e-cigarettes from the market except for tobacco and menthol. Subsequent studies found that some former users of the now-removed flavoured JUUL pods (especially cool mint) switched to menthol-flavoured JUUL pods with similar satisfaction.Methods We quantified menthol, nicotine, propylene glycol (PG) and vegetable glycerol (VG) in JUUL pod samples (Menthol, Classic Menthol and Cool Mint) that were purchased in 2017, 2018 and 2020 (only Menthol) to evaluate composition differences before and after the enforcement policy. We also analysed the samples to detect other cooling agents using a screening gas chromatography-mass spectrometry headspace method that we developed for this purpose.Results Nicotine and menthol concentrations were significantly higher in 2020 products than in products from prior years. Moreover, other cooling agents varied across pods. The PG/VG volume ratio was 27/63 in all pods examined.Conclusion This study shows that concentrations of nicotine and menthol in JUUL products procured over a three year period decreased over time. The findings highlight the challenges of reconstructing product characteristics at the time of manufacture and time of consumption, and suggests the utility of an ENDS product bank that could preserve samples for retrospective studies. They also underscore that manufacturers should be required to release to the public and the scientific community what they know about time- and temperature-dependent degradation of their products.The data supporting the findings are available within the article. %U https://tobaccocontrol.bmj.com/content/tobaccocontrol/31/Suppl_3/s234.full.pdf