Official methods for the collection of data on constituents

ItemConstituentOfficial method
1(a) Nicotine; (b) nornicotine; (c) anabasine; (d) myosmine; (e) anatabineOfficial method T-301, Determination of alkaloids in whole tobacco
2AmmoniaOfficial method T-302, Determination of ammonia in whole tobacco
3(a) Glycerol; (b) propylene glycol; (c) triethylene glycolOfficial method T-304, Determination of humectants in whole tobacco
4.(a) Nickel; (b) lead; (c) cadmium; (d) chromium; (e) arsenic; (f) selenium; (g) mercuryOfficial method T-306, Determination of Ni, Pb, Cd, Cr, As, Se and Hg in whole tobacco
5.Benzo[a]pyreneOfficial method T-307, Determination of benzo[a]pyrene in whole tobacco
6NitrateOfficial method T-308, Determination of nitrate from whole tobacco
7(a) N-nitrosonornicotine; (b) 4-(N-nitrosomethylamino)-l-(3-pyridyl)-1-butanone; (c) N-nitrosoanatabine; (d) N-nitrosoanabasineOfficial method T-309, Determination of nitrosamines in whole tobacco
8TriacetinOfficial method T-311, Determination of triacetin in whole tobacco
9Sodium propionateOfficial method T-312, Determination of sodium propionate in whole tobacco
10Sorbic acidOfficial method T-313, Determination of sorbic acid in whole tobacco
11Eugenol [2-methoxy-4-(2-propenyl)-phenol]Official method T-314, Determination of eugenol in whole tobacco