E-cigarette product information and human pulmonary fibroblast cytotoxicity data of the fluids and aerosols
| Inv. no | Refill fluid (company) | Flavour category | Nicotine (mg/mL) | Solvent | Aerosol (%) | RF redo IC50 (%) | <70% of CN | |||
| NOAEL | LOAEL | IC50 | A | RF | ||||||
| 6 | Valencia (R) | Fruit | 18 | G, V | 1.008 | 3.360 | >3 | >1 | No | No |
| 34 | JC Original (J) | Tobacco | 11 | P, V | 0.031 | 0.092 | >3 | >1 | No | No |
| 8 | Arctic Menthol (J) | Mint/tobacco | 18 | P, V, G | 0.744 | 2.479 | >2 | >1 | Yes | Yes |
| 10 | Chocolate Truffle (J) | Chocolate/creamy/tob. | 18 | P, V, G | 0.721 | 2.403 | >2 | 2.038 | Yes | Yes |
| 13 | JC Original (J) | Tobacco | 18 | P, V, G | 0.277 | 0.830 | >2 | >1 | No | No |
| 15 | Simply Strawberry (J) | Fruit | 18 | P, V, G | 0.250 | 0.750 | >2 | >1 | Yes | Yes |
| 16 | Summer Peach (J) | Fruit | 18 | P, V, G | 0.768 | 2.559 | >2 | >1 | No | No |
| 23 | Menthol Arctic (F) | Mint/menthol | 0 | U | 0.659 | 2.195 | >2 | 1.439 | No | Yes |
| 32 | Propylene Glycol (F) | – | – | P | 0.236 | 0.707 | >2 | 2.109 | Yes | Yes |
| 12 | French Vanilla (J) | Nutty/creamy/tob. | 18 | P, V, G | 0.507 | 1.689 | >1 | >1 | No | No |
| 14 | Mint Chocolate (J) | Mint/chocolate | 18 | P, V, G | 0.190 | 0.570 | >1 | >1 | No | No |
| 17 | Tennessee Cured (J) | Tobacco | 18 | P, V, G | 0.519 | 1.730 | >1 | >1 | No | No |
| 27 | Caramel (F) | Creamy/buttery | 6 | U | 0.495 | 1.649 | >1 | >1 | Yes | Yes |
| 40 | Caramel (G.S.) | Creamy/buttery | 18 | U | 0.172 | 0.516 | >1 | >1 | Yes | Yes |
| 9 | Black Cherry (J) | Fruit/tobacco | 18 | P, V, G | 0.643 | 2.142 | 2.036 | >1 | Yes | Yes |
| 18 | Bubblegum (F) | Candy | 24 | U | 0.660 | 2.201 | 1.635 | >1 | Yes | Yes |
| 11 | Espresso (J) | Coffee/creamy/tob. | 18 | P, V, G | 0.727 | 2.423 | 1.587 | >1 | Yes | Yes |
| 21 | Caramel (F) | Creamy/buttery | 0 | U | 0.233 | 0.698 | 1.499 | 0.560 | Yes | Yes |
| 31 | Tennessee Cured (J) | Tobacco | 11 | P, V | 0.704 | 2.347 | 1.420 | >1 | Yes | Yes |
| 24 | Pure Nicotine Liquid (F) | – | U* | P | 0.655 | 2.184 | 1.385 | 0.750 | Yes | Yes |
| 3 (73) | Marcado (R) | Spiced/tobacco | 18 | G, V | 0.850 | 2.834 | 1.382 | 0.210 | Yes | Yes |
| 2 | Island (R) | Fruit | 18 | G, V | 0.268 | 0.805 | 1.174 | 1.456 | Yes | Yes |
| 41 | Butterscotch (F) | Creamy/buttery | 0 | U | 0.213 | 0.639 | 1.020 | 0.700 | Yes | Yes |
| 5 | Tennessee Cured (R) | Tobacco | 18 | G, V | 0.525 | 1.749 | 0.875 | 1.101 | Yes | Yes |
| 25 | Vanilla Tahity (F) | Creamy/buttery | 0 | U | 0.160 | 0.479 | 0.563 | 0.640 | Yes | Yes |
| 33 | Vegetable Glycerin (F) | – | – | V | 0.152 | 0.457 | 0.517 | >1 | Yes | No |
| 1 | Domestic (R) | Tobacco | 18 | G, V | 0.148 | 0.443 | 0.453 | >1 | Yes | No |
| 7 | Wisconsin Frost (R) | Mint/tobacco | 18 | G, V | 0.170 | 0.511 | 0.426 | >1 | Yes | No |
| 22 | Cinnamon Ceylon (F) | Spiced/cinnamon | 0 | U | 0.092 | 0.306 | 0.408 | 0.020 | Yes | Yes |
| 19 | Butterfinger (F) | Creamy/buttery | 24 | U | 0.043 | 0.143 | 0.333 | >1 | Yes | No |
| 28 | Caramel (F) | Creamy/buttery | 6 | V | 0.044 | 0.146 | 0.119 | >1 | Yes | No |
| 30 | Butterscotch (F) | Creamy/buttery | 0 | V | 0.044 | 0.146 | 0.105 | >1 | Yes | No |
| 29 | Butterscotch (F) | Creamy/buttery | 6 | V | 0.005 | 0.016 | 0.023 | >1 | Yes | No |
| 26 | Caramel (F) | Creamy/buttery | 0 | V | 0.004 | 0.014 | 0.021 | >1 | Yes | No |
| 4 | Swiss Dark (R) | Chocolate/creamy/tob. | 18 | G, V | 0.004 | 0.012 | 0.018 | 0.303 | Yes | Yes |
The column titled <70% of CN denotes a response in the MTT (3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide) assay that fell below 70% of the control. Solvent (P, G or V) was recorded as it appears on the company’s label.
*Products are ordered based on the aerosol inhibitory concentration at 50% (IC50) values.
A, aerosol; CN, control; F, Freedom Smoke USA; G, glycerol; G.S. Global Smoke; Inv. no, inventory number; J, Johnson Creek; LOAEL, lowest observed adverse effect levels; NOAEL, no observed adverse effect levels; P, propylene glycol; R, Red Oak; RF, refill fluid; tob, tobacco; U, unknown solvent type; U*, unknown nicotine concentration; V, vegetable glycerin.